Issue 1, 2002
From the journal:

Lab on a Chip

Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor†

Ken-ichi Kanno,*a   Hideaki Maeda,b   Showta Izumo,a   Masakazu Ikuno,a   Kazuhito Takeshita,a   Asuka Tashiroa  and  Masayuki Fujiiac  

* Corresponding authors

a Department of Bio-Environmental Chemistry, Kyushu School of Engineering, Kinki University, Kayanomori 11-6, Iizuka-shi, Fukuoka, Japan

b National Institute of Industrial Science and Technology (AIST), Suku-machi 807-1, Tosu, Saga, Japan

c Henkel Kindai Laboratories (HKL), Center of Advanced Technology, Kayanomori 11-6, Iizuka-shi, Fukuoka, Japan

Abstract

Glycosidase-promoted hydrolysis was performed in a microreaction channel. The result was compared with the reaction in a micro-test tube. Transgalactosylation on p-nitrophenyl-2-acetamide-2-deoxy-β-D-glucopyranoside was also examined in a microreaction channel, because transglycosylation is a useful method for oligosaccharide synthesis. We examined both the forward reaction, i.e., hydrolysis, and the reverse reaction, i.e., transglycosylation, in the microreaction channel. The results showed that both hydrolysis and transglycosylation were enhanced in the microreaction channel compared with the batch reaction.

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Article information

DOI
https://doi.org/10.1039/B108741J
Article type
Paper
Submitted
25 Sep 2001
Accepted
12 Dec 2001
First published
17 Jan 2002

Lab Chip, 2002,2, 15-18

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Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor

K. Kanno, H. Maeda, S. Izumo, M. Ikuno, K. Takeshita, A. Tashiro and M. Fujii, Lab Chip, 2002, 2, 15 DOI: 10.1039/B108741J

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