Fluorescence quenching high-performance thin-layer chromatographic analysis utilizing a scientifically operated charge-coupled device detector

Abstract

A scientifically operated charge-coupled device detector combined with fluorescence quenching high-performance thin-layer chromatographic plates was employed for the detection of organic compounds, The plates were excited with 254 nm light from a mercury lamp, and quantitative information was obtained from organic compounds that absorbed the optimum conditions for detection. The linear dynamic range, sensitivity, and reproducibility of the system were evaluted by quantitative analysis of famotidine, acetaminophen, caffeine, and acetylsalicylic acid. The detection limits of the system were found to be in the nanogram range.