Issue 4, 1999

Sample preparation and HPLC separation approaches to speciation analysis of selenium in yeast by ICP-MS

Abstract

Eight solid-liquid extraction procedures were evaluated for the recovery of selenium species from yeast. Speciation of Se in the extracts was characterized by different types of HPLC, including size-exclusion, anion-exchange and reversed-phase chromatography with ICP-MS detection. The results obtained depended critically on the sample preparation procedure used. Leaching with water and with methanol led only to 10-20% recoveries of Se, split into eight compounds, among which Se(IV) and selenomethionine could be identified. Leaching with pectinolytic enzymes released an additional 20% of selenomethionine. Leaching with sodium dodecyl sulfate solution allowed the solubilization of a selenoprotein that accounted for ca. 30% of the total Se present. Leaching with proteolytic enzymes led to recoveries of Se above 85%, the majority as selenomethionine. Hydrolysis of the yeast with tetramethylammonium hydroxide solubilized the sample completely but the Se species present were entirely degraded to selenomethionine and inorganic selenium. A sequential leaching procedure is proposed for the evaluation of selenium speciation in yeast without the need for a coupled technique.

Article information

Article type
Paper

J. Anal. At. Spectrom., 1999,14, 645-650

Sample preparation and HPLC separation approaches to speciation analysis of selenium in yeast by ICP-MS

C. Casiot, J. Szpunar, R. Łobiński and M. Potin-Gautier, J. Anal. At. Spectrom., 1999, 14, 645 DOI: 10.1039/A809027K

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