Protein film cryovoltammetry: demonstrations with a 7Fe ([3Fe–4S] + [4Fe–4S]) ferredoxin
Abstract
Low-temperature protein film voltammetry, demonstrated here by studies of Fe–S clusters in the 7Fe ferredoxin from Thermoplasma acidophilum, offers a novel way to investigate active site redox reactions, allowing resolution of complex transformations and transient states, and providing an immediate and sensitive gauge of solvent effects.