High esterolytic activity of a novel water-soluble polymer catalyst imprinted by a transition-state analogue

Abstract

A novel water-soluble polymer catalyst containing a L-histidyl group as a catalytic site, prepared by imprinting of a transition-state analogue of phenyl 1-benzyloxycarbonyl-3-methylpentyl phosphonate, accelerates the substrate-specific esterolysis of p-nitrophenyl N-(benzyloxycarbonyl)-L-leucinate (Z-L-Leu-PNP) in 10 vol% Me₂O–Tris buffer (pH 7. 15) at 293–308 K.