Syntheses of novel 25-hydroxyvitamin D3 haptens having chemical bridges at the C-11α position

Abstract

The serum or plasma levels of 25-hydroxyvitamin D₃1a is useful for the evaluation of vitamin D status in various clinical or nutritional disorders. To obtain antibodies to compound 1a which are highly specific and useful for development of immunoassays, two novel haptenic derivatives, 11α-(3-carboxypropionyloxy)-25-hydroxyvitamin D₃2a and 11α-(4-carboxybutyryloxy)-25-hydroxyvitamin D₃2b were synthesized each in 21 steps from 11α-hydroxydehydroepiandrosterone 3.