Kinetics and thermodynamics of iodide binding to aquacobalamin in methanol–water solvent mixtures
Abstract
The rates and equilibrium constants for the reaction of aquacobalamin with iodide were measured as a function of solvent composition using methanol–water solutions. The solvent effects are consistent with a reaction that proceeds via the formation of an outer-sphere complex which is more stable for the cobalamin–methanol than for the cobalamin–water complex.