Liquid chromatography with series dual-electrode electrochemical detection for riboflavin

Abstract

A method for the selective detection of riboflavin (vitamin B₂) using liquid chromatography with a series dual-electrode electrochemical detector is described. The upstream electrode was held at –0.4 V (versus SCE) with the downstream electrode at +0.1 V (versus SCE). The linear range is 4 ng–2.6 µg and the limit of detection is 4 ng. The interference of 13 different types of vitamin on the absorbance or electrochemical detection of vitamin B₂ was studied. No interference was found for the detection of vitamin B₂ using the series dual-electrode detector. The method is convenient and rapid and has high selectivity. The application of the method to the detection of vitamin B₂ in tablets is also described.