High-performance liquid chromatographic determination of cinnamaldehyde

Abstract

A sensitive method for the determination of cinnamaldehyde is described, using high-performance liquid chromatography with spectrofluorimetric detection. Cinnamaldehyde was first reacted with 4,5-dimethyl-o-phenylenediamine in an acidic medium and the resulting fluorescent benzimidazole derivative was analysed on a reversed-phase column with 0.02 M acetate buffer (pH 4.0)-methanol (1 + 1) containing 5 mmol l^–1 dodecyltrimethylammonium bromide as the mobile phase. Cinnamaldehyde was determined in aqueous solution in the range 0.001–30 µg ml^–1.