Stereochemical investigation of the α-ketoglutarate-dependent 3′-hydroxylation in cephalosporin biosynthesis
Abstract
Using samples containing D,L-(3S,4S)- and (3S,4R)-[4-2H,3H]valine, the stereochemical course of the α-ketoglutarate-dependent 3′-hydroxylation of deactoxycephalosporin C (2) to deacetylcephalosporin C(3) has been determined to be retention.