Studies in terpenoid biosynthesis. Part 28. The acetate and mevalonate labelling patterns of the steroid, demethoxyviridin

Abstract

The enrichment and labelling patterns of demethoxyviridin, biosynthesized by Nodulisporium hinnuleum from [1-¹³C]-, [1,2-¹³C₂]-acetate, [2-¹³C]- and [5-¹³C]-mevalonate have been used to define the isoprene units in this metabolite and are consistent with a triterpenoid origin. The number and location of the hydrogen atoms originating from acetate and the 2-, 4-, and 5-positions of mevalonate, have been determined by a combination of ³H : ¹⁴C ratio and ²H n.m.r. studies.