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Issue 17, 2015
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The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

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Abstract

There is a recognized and growing need for rapid and efficient cell assays, where the size of microfluidic devices lend themselves to the manipulation of cellular populations down to the single cell level. An exceptional way to analyze cells independently is to encapsulate them within aqueous droplets surrounded by an immiscible fluid, so that reagents and reaction products are contained within a controlled microenvironment. Most cell encapsulation work has focused on the development and use of passive methods, where droplets are produced continuously at high rates by pumping fluids from external pressure-driven reservoirs through defined microfluidic geometries. With limited exceptions, the number of cells encapsulated per droplet in these systems is dictated by Poisson statistics, reducing the proportion of droplets that contain the desired number of cells and thus the effective rate at which single cells can be encapsulated. Nevertheless, a number of recently developed actively-controlled droplet production methods present an alternative route to the production of droplets at similar rates and with the potential to improve the efficiency of single-cell encapsulation. In this critical review, we examine both passive and active methods for droplet production and explore how these can be used to deterministically and non-deterministically encapsulate cells.

Graphical abstract: The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

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Publication details

The article was received on 03 Jun 2015, accepted on 21 Jul 2015 and first published on 30 Jul 2015


Article type: Critical Review
DOI: 10.1039/C5LC00614G
Citation: Lab Chip, 2015,15, 3439-3459
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    The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

    D. J. Collins, A. Neild, A. deMello, A. Liu and Y. Ai, Lab Chip, 2015, 15, 3439
    DOI: 10.1039/C5LC00614G

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