Jump to main content
Jump to site search

Issue 18, 2014
Previous Article Next Article

Droplet optofluidic imaging for λ-bacteriophage detection via co-culture with host cell Escherichia coli

Author affiliations

Abstract

Bacteriophages are considered as attractive indicators for determining drinking water quality since its concentration is strongly correlated with virus concentrations in water samples. Previously, bacteriophage detection was based on a plague assay that required a complicated labelling technique and a time-consuming culture assay. Here, for the first time, a label-free bacteriophage detection is reported by using droplet optofluidic imaging, which uses host-cell-containing microdroplets as reaction carriers for bacteriophage infection due to a higher contact ratio. The optofluidic imaging is based on the effective refractive index changes in the microdroplet correlated with the growth rate of the infected host cells, which is highly sensitive, i.e. can detect one E. coli cell. The droplet optofluidic system is not only used in drinking water quality monitoring, but also has high potential applications for pathogenic bacteria detection in clinical diagnosis and food industry.

Graphical abstract: Droplet optofluidic imaging for λ-bacteriophage detection via co-culture with host cell Escherichia coli

Back to tab navigation

Supplementary files

Publication details

The article was received on 12 Jan 2014, accepted on 12 Jun 2014 and first published on 12 Jun 2014


Article type: Paper
DOI: 10.1039/C4LC00042K
Citation: Lab Chip, 2014,14, 3519-3524
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Droplet optofluidic imaging for λ-bacteriophage detection via co-culture with host cell Escherichia coli

    J. Q. Yu, W. Huang, L. K. Chin, L. Lei, Z. P. Lin, W. Ser, H. Chen, T. C. Ayi, P. H. Yap, C. H. Chen and A. Q. Liu, Lab Chip, 2014, 14, 3519
    DOI: 10.1039/C4LC00042K

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements