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Volume 172, 2014
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Comparison of carbon materials as electrodes for enzyme electrocatalysis: hydrogenase as a case study

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Abstract

We present a study of electrocatalysis by an enzyme adsorbed on a range of carbon materials, with different size, surface area, morphology and graphitic structure, which are either commercially available or prepared via simple, established protocols. We choose as our model enzyme the hydrogenase I from E. coli (Hyd-1), which is an active catalyst for H2 oxidation, is relatively robust and has been demonstrated in H2 fuel cells and H2-driven chemical synthesis. The carbon materials were characterised according to their surface area, surface morphology and graphitic character, and we use the electrocatalytic H2 oxidation current for Hyd-1 adsorbed on these materials to evaluate their effectiveness as enzyme electrodes. Here, we show that a variety of carbon materials are suitable for adsorbing hydrogenases in an electroactive configuration. This unified study provides insight into selection and design of carbon materials for study of redox enzymes and different applications of enzyme electrocatalysis.

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Publication details

The article was received on 04 Apr 2014, accepted on 11 Apr 2014 and first published on 14 Aug 2014


Article type: Paper
DOI: 10.1039/C4FD00058G
Citation: Faraday Discuss., 2014,172, 473-496
  • Open access: Creative Commons BY license
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    Comparison of carbon materials as electrodes for enzyme electrocatalysis: hydrogenase as a case study

    J. Quinson, R. Hidalgo, P. A. Ash, F. Dillon, N. Grobert and K. A. Vincent, Faraday Discuss., 2014, 172, 473
    DOI: 10.1039/C4FD00058G

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