Issue 15, 2024, Issue in Progress
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RSC Advances

In situ imaging of mRNA transcripts based on split-aptamer and split protein in living cells†

Yan Peng, ORCID logo ‡*ab   Mengqi Li,‡a   Fuyu Gong,*ab   Xiaofei Liu,ac   Jirou Xionga  and  Keran Wanga  

* Corresponding authors

a The Affiliated First Hospital of Fuyang Normal University, Fuyang Normal University, Fuyang, Anhui, P.R. China
E-mail: pengyan@fynu.edu.cn, gong2022@fynu.edu.cn

b Fuyang Women and Children's Hospital, Fuyang, Anhui, P.R. China

c Fuyang People's Hospital, Fuyang, Anhui, P.R. China

Abstract

Messenger RNA (mRNA) is an essential component of cell development and growth. However, the detection of endogenous mRNA in living cells is currently limited. To address this issue, we have developed a novel strategy that comprises split-aptamer and split fluorescent protein dual-color, “turn-on” probes that specifically target mRNA with complementary sequences. Our split-aptamer and split-protein-initiated fluorescence complementation (sAPiFC) approach for live-cell imaging has demonstrated selectivity, stability, and capability for targeting various mRNAs.

Graphical abstract: In situ imaging of mRNA transcripts based on split-aptamer and split protein in living cells

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Article information

DOI
https://doi.org/10.1039/D3RA08475B
Article type
Paper
Submitted
12 Dec 2023
Accepted
20 Mar 2024
First published
26 Mar 2024
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2024,14, 10146-10151

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In situ imaging of mRNA transcripts based on split-aptamer and split protein in living cells

Y. Peng, M. Li, F. Gong, X. Liu, J. Xiong and K. Wang, RSC Adv., 2024, 14, 10146 DOI: 10.1039/D3RA08475B

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