Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor

Cheryl S.Y. Yeap; Thanyarat Chaibun; Su Yin Lee; Bin Zhao; Yuan Jan; Chan La-o-vorakiat; Werasak Surareungchai; Shiping Song; Benchaporn Lertanantawong

doi:10.1039/D1CC05181D

Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor†

Cheryl S.Y. Yeap,

^abc Thanyarat Chaibun,^ac Su Yin Lee,

^d Bin Zhao,^ef Yuan Jan,

^eg Chan La-o-vorakiat,

^c Werasak Surareungchai,^ch Shiping Song^e and Benchaporn Lertanantawong

*^ac

Author affiliations

* Corresponding authors

^a Biosensors Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom, Thailand
E-mail: benchaporn.ler@mahidol.ac.th

^b Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Studentská 1402/2, 461 17 Liberec, Czech Republic

^c Pilot Plant and Development Training Institute, Nanoscience and Nanotechnology Research Cluster, King Mongkut's University of Technology Thonburi (KMUTT), Bang Khun Thian Campus, Bangkok 10150, Thailand

^d Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio) and Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Kedah, Malaysia

^e Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China

^f Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA

^g College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

^h School of Bioresources & Technology, King Mongkut's University of Technology Thonburi, Thailand

Abstract

We report a highly sensitive and selective multiplex assay by empowering an electrochemical DNA sensor with isothermal rolling circle amplification. The assay could simultaneously detect and discriminate three common entero-pathogens in a single reaction, with femtomolar sensitivity. It is useful for field- or resource-limited settings.

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Article information

DOI: https://doi.org/10.1039/D1CC05181D
Article type: Communication
Submitted: 14 Sep 2021
Accepted: 19 Oct 2021
First published: 20 Oct 2021

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Chem. Commun., 2021,57, 12155-12158

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Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor

C. S.Y. Yeap, T. Chaibun, S. Y. Lee, B. Zhao, Y. Jan, C. La-o-vorakiat, W. Surareungchai, S. Song and B. Lertanantawong, Chem. Commun., 2021, 57, 12155 DOI: 10.1039/D1CC05181D

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Chemical Communications

Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor†

Abstract

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Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor

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