An aptasensor for the detection of ampicillin in milk using a personal glucose meter

Abstract

Antibiotic residues in foods have aroused wide public concern because of their potential side-effects. It is imperative to develop a simple, accurate and reliable method for the detection of antibiotic residues in foods. In this paper, we report a novel, facile and sensitive method for the detection of ampicillin in milk using a commercial personal glucose meter (PGM). Magnetic beads (MBs) were employed as the platform, an ampicillin aptamer was used as the recognition element and streptavidin was utilized as the bridge to link invertase and the aptamer. After the hydrolysis of sucrose to glucose, the concentration of glucose was quantitatively measured using the PGM. The difference of PGM signals with and without addition of ampicillin exhibits a good linear correlation with the logarithm of ampicillin concentrations in the range of 2.5 × 10⁻¹⁰ mol L⁻¹ to 1.0 × 10⁻⁷ mol L⁻¹ with a detection limit of 2.5 × 10⁻¹⁰ mol L⁻¹ (S/N = 3). Finally, the proposed method was successfully applied for the detection of ampicillin residue in milk.