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Upregulated has-miR-4516 as a potential biomarker for early diagnosis of dust-induced pulmonary fibrosis in patients with pneumoconiosis

Abstract

Background: Pulmonary fibrosis (PF) is a major clinical symptom in patients with pneumoconiosis; however, due to the absence of reliable and early biomarkers, microRNAs have recently emerged as potential candidates for identification. Objectives: The aim of our study was to discover the potential of PF-specific circulating microRNAs for early biomarkers among patients with pneumoconiosis. Methods: Four dust-exposed patients with PF and four matched healthy individuals (not exposed to dust) were recruited for the study. microRNA profiling were identified by micro-array and bioinformatics method. Gene Ontology (GO) analysis was used to identify potential biological or molecular processes modulated by these miRNAs. Kyoto Encyclopedia of Genes and Genomes pathway(KEGG) analysis was used to identify potentially involved signaling pathways. MiRNA-mRNA-binding network analysis was employed to identify genes potentially targeted by the miRNAs. Results:1,079 miRNAs were discovered, of which 406 were up-regulated and 117 down-regulated in PF patients. 32 miRNAs were up-regulated by > 4-fold and 17 were down-regulated by>0.5 fold. GO analysis identified the biological processes affected by anatomical structure development, hemophilic cell adhesion and cell-cell adhesion via plasma membrane proteins. Target prediction software showed that serum has-miR-4516 targeted genes encoding basonuclin 2, inhibitor of growth family member 4, the potassium voltage-gated channel, and “sha-1-related subfamily member 1” proteins. QRT-PCR revealed that has-miR-4516 was a potential biomarker of PF progression in patients with pneumoconiosis. Conclusions: microarray analysis effectively identified potentially prognostic miRNAs in patients with PF and bioinformatics approaches identified the signaling pathways involved and potentially targeted genes, facilitating more specific future studies. has-miR-4516 is a potential new biomarker of PF progression in patients with pneumoconiosis. Future studies are required to ascertain the biological function of has-miR-4516. This is a pilot work that paves the way for a further functional study of the underlying regulatory mechanisms.

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Publication details

The article was received on 21 Jan 2018, accepted on 08 Feb 2018 and first published on 08 Feb 2018


Article type: Paper
DOI: 10.1039/C8TX00031J
Citation: Toxicol. Res., 2018, Accepted Manuscript
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    Upregulated has-miR-4516 as a potential biomarker for early diagnosis of dust-induced pulmonary fibrosis in patients with pneumoconiosis

    R. huang, T. yu, Y. li and J. hu, Toxicol. Res., 2018, Accepted Manuscript , DOI: 10.1039/C8TX00031J

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