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Issue 3, 2018
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Evaluation of the toxic potential of arecoline toward the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9

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Abstract

Arecoline is the key component of areca nut and has been suggested as a carcinogenic agent. In the present study, the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9 were allowed to feed on a diet having 5, 10, 20, 40 and 80 μM arecoline for 24 h. After the completion of 24 h, the larvae were subjected to ONPG assay, X-gal staining, trypan blue exclusion test, oxidative stress markers, and apoptotic and comet assays. A dose-dependent increase in the β-galactosidase activity, tissue damage, glutathione-S-transferase (GST) activity, lipid peroxidation assay, monoamine oxidase (MAO), caspase-9 and 3, protein carbonyl content (PCC), apoptotic index, and DNA damage and decrease in glutathione (GSH) content, delta aminolevulinic acid dehydrogenase (δ-ALA-D), and acetylcholinesterase (AChE) activity were observed in the larvae exposed to 20, 40 and 80 μM arecoline. The results suggest that arecoline is toxic at 20, 40, and 80 μM toward the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9. Arecoline did not show any toxic effects at 5 and 10 μM.

Graphical abstract: Evaluation of the toxic potential of arecoline toward the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9

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Publication details

The article was received on 13 Nov 2017, accepted on 22 Feb 2018 and first published on 20 Mar 2018


Article type: Paper
DOI: 10.1039/C7TX00305F
Citation: Toxicol. Res., 2018,7, 432-443
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    Evaluation of the toxic potential of arecoline toward the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9

    B. Shakya and Y. H. Siddique, Toxicol. Res., 2018, 7, 432
    DOI: 10.1039/C7TX00305F

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