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Issue 3, 2018
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In silico identification of protein targets for chemical neurotoxins using ToxCast in vitro data and read-across within the QSAR toolbox

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Abstract

There are many mechanisms of neurotoxicity that are initiated by the interaction of chemicals with different neurological targets. Under the U.S. Environmental Protection Agency's ToxCast program, the biological activity of thousands of chemicals was screened in biochemical and cell-based assays in a high-throughput manner. Two hundred sixteen assays in the ToxCast screening database were identified as targeting a total of 123 proteins having neurological functions according to the Gene Ontology database. Data from these assays were imported into the Organization for Economic Co-operation and Development QSAR Toolbox and used to predict neurological targets for chemical neurotoxins. Two sets of data were generated: one set was used to classify compounds as active or inactive and another set, composed of AC50s for only active compounds, was used to predict AC50 values for unknown chemicals. Chemical grouping and read-across within the QSAR Toolbox were used to identify neurologic targets and predict interactions for pyrethroids, a class of compounds known to elicit neurotoxic effects in humans. The classification prediction results showed 79% accuracy while AC50 predictions demonstrated mixed accuracy compared with the ToxCast screening data.

Graphical abstract: In silico identification of protein targets for chemical neurotoxins using ToxCast in vitro data and read-across within the QSAR toolbox

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Publication details

The article was received on 10 Oct 2017, accepted on 10 Jan 2018 and first published on 12 Mar 2018


Article type: Paper
DOI: 10.1039/C7TX00268H
Citation: Toxicol. Res., 2018,7, 423-431
  • Open access: Creative Commons BY-NC license
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    In silico identification of protein targets for chemical neurotoxins using ToxCast in vitro data and read-across within the QSAR toolbox

    Y. G. Chushak, H. W. Shows, J. M. Gearhart and H. A. Pangburn, Toxicol. Res., 2018, 7, 423
    DOI: 10.1039/C7TX00268H

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