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Growth Factor-Free Salt-leached Silk Scaffolds to Differentiate Endothelial Cells

Abstract

Recently, controllable kinetic assembly was introduced into the salt-leaching process with silk proteins to form scaffolds, which achieved improvement in tuning the micro-structural and mechanical properties. Here, more control of the kinetic assembly of silk in the process was integrated into salt-leaching process, resulting in significant mechanical modification of the scaffolds generated. Both glycerol additions and treatment to concentrate the protein were used to tune hydrophilic interactions during aqueous solution processing and to reduce beta-sheet formation during the salt-leaching process. These new scaffolds showed gradient changes in elastic modulus in the range of 0.9 to 7.9 kPa. Bone marrow mesenchymal stem cells grew well and showed endothelial differentiation behavior on the scaffolds with optimized stiffness. These results indicated that the introduction of silk kinetic assembly provides an additional option for the control of porous silk scaffold properties.

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Publication details

The article was received on 17 Apr 2018, accepted on 11 Jun 2018 and first published on 12 Jun 2018


Article type: Paper
DOI: 10.1039/C8TB01001C
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Growth Factor-Free Salt-leached Silk Scaffolds to Differentiate Endothelial Cells

    L. Xiao, C. Zhu, Z. Ding, S. Liu, D. Yao, Q. Lu and D. Kaplan, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB01001C

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