Jump to main content
Jump to site search


Highly Sensitive Fluorescent Quantification of Intracellular Telomerase Activity by Repeat G-rich DNA Enhanced Silver Nanocluster

Abstract

As an important biomarker for early cancer diagnosis and a valuable therapeutic target, the detection and monitoring of telomerase activity has attracted extensive attention. Herein, we constructed a novel fluorescent reporter to detect the intracellular telomerase activity based on the guanines enhanced sliver nanoclusters (Ag NCs). In this system, telomerase substrate (Ts) was designed to be extended by telomerase to give the repeat TTAGGG sequence as G-rich DNA, which was complementary to nine bases sequence to form a self-hairpin structure. The proximity of G-rich DNA to the as-prepared Ag NCs resulted into a 13-fold enhancement of fluorescence intensity due to the electron transfer from guanines to the Ag NCs. Therefore the fluorescent probe could realize the detection of telomerase activity. Furthermore, the probe was successfully used to distinguish normal cells from cancer cells, and monitor the real time telomerase activity response upon treating with inhibiting model drug. This detection technique is simple, with excellent biocompatibility, inexpensive and expands the applications of Ag NCs probe.

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 Mar 2018, accepted on 11 May 2018 and first published on 14 May 2018


Article type: Paper
DOI: 10.1039/C8TB00801A
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
  •   Request permissions

    Highly Sensitive Fluorescent Quantification of Intracellular Telomerase Activity by Repeat G-rich DNA Enhanced Silver Nanocluster

    S. Huang, H. Yao, W. Wang, J. Zhang and J. Zhu, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00801A

Search articles by author

Spotlight

Advertisements