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Nanoparticle-supported polymer brushes for temperature-regulated glycoprotein separation: investigation of structure-function relationship

Abstract

In this work, we synthesized a series of nanoparticle-supported boronic acid polymer brushes for affinity separation of glycoproteins. The polymer brushes were prepared by surface-initiated atom transfer radical polymerization of glycidyl methacrylate and N-isopropylacrylamide, followed by stepwise modification of the pendant as well as the end functional groups to introduce the boronic acid moieties through Cu(I)-catalyzed alkyne-azide cycloaddition reaction. We investigated the impact of the polymer structure on glycoprotein binding under different pH and temperature conditions, and established new methods that allow glycoproteins to be more easily isolated and recovered with minimal alternation of solvent composition. Our experimental results suggest that for separation of glycoproteins, terminal boronic acids located at the end of polymer chains play the most important role. The thermo-responsibility of the new affinity adsorbents, in addition to the high capacity for glycoprotein binding (120 mg ovalbumin/g adsorbent), provides a convenient means to realizing simplified bioseparation not only for glycoproteins, but also for other carbohydrate-containing biological molecules.

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Publication details

The article was received on 06 Mar 2018, accepted on 03 May 2018 and first published on 03 May 2018


Article type: Paper
DOI: 10.1039/C8TB00627J
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Nanoparticle-supported polymer brushes for temperature-regulated glycoprotein separation: investigation of structure-function relationship

    H. Ma, L. Jiang, S. Hajizadeh, H. Gong, B. Lu and L. Ye, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00627J

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