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Mussel adhesive protein fused with VE-cadherin domain specifically triggers endothelial cell adhesion

Abstract

The endothelium is the only known completely non-thrombogenic material. In the present study, a strategy to mimic the adhesive interactions of endothelial cells (ECs) to alter the vascular microenvironment was established and applied to direct cells’ behavior. To facilitate the regeneration of a functional endothelium in vascular lesions, we designed a recombinant mussel foot protein (Mfp-5) fused with the VE-cadherin extracellular domain EC1-2, termed VE-M. Surface coating analysis showed that recombinant VE-M successfully formed a coating on substrate materials with uniform nanorods, low roughness, and sufficient hydrophilicity. We then evaluated the effects of VE-M on adhesion of ECs and the capture of endothelial progenitor cells (EPCs). The result demonstrated that VE-M efficiently promoted the adhesion of ECs and EPCs. The number of ECs and EPCs on VE-M was 5.5- and 1.8-fold higher, respectively, than that on bare 316L SS under static conditions, whereas there was no significant difference in the number of captured smooth muscle cells (SMCs) between VE-M and other substrates. In addition, the number of EPCs captured by VE-M was approximately four times of that captured by 316L SS under dynamic conditions. More importantly, the result of neutralization test indicated that VE-M specifically triggers ECs adhesion via the interaction of VE-cadherin EC1-2. Further investigation showed that VE-M significantly increased the levels of endogenous VE-cadherin in HUVECs, as well as the endothelial eNOS content, with little or no endothelial inflammation. Our results showed that VE-M could be a promising biomimetic modification for accelerating endothelialization and vascularization in tissue engineering.

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Publication details

The article was received on 25 Feb 2018, accepted on 11 May 2018 and first published on 16 May 2018


Article type: Paper
DOI: 10.1039/C8TB00526E
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Mussel adhesive protein fused with VE-cadherin domain specifically triggers endothelial cell adhesion

    D. Yang, J. Qiu, N. Xu, Y. Zhao, T. Li, Q. Ma, J. Huang and G. Wang, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00526E

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