Issue 22, 2018

Sensitive and specific detection of clinical bacteria via vancomycin-modified Fe3O4@Au nanoparticles and aptamer-functionalized SERS tags

Abstract

A sensitive surface-enhanced Raman scattering (SERS) platform for the simultaneous detection of two pathogenic bacteria on the basis of dual recognition by vancomycin and aptamers is reported for the first time, using Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) as target bacteria. This dual-recognition platform was developed using vancomycin-modified Fe3O4@Au magnetic nanoparticles (Fe3O4@Au-Van MNPs) for broad-spectrum recognition and efficient bacterial enrichment, and aptamer-functionalized SERS tags for the specific and sensitive quantification of target bacteria in authentic specimens. Our results showed that the Fe3O4@Au-Van MNPs allow the rapid capture of bacteria from complex solutions with a capture efficiency as high as 88.89% for S. aureus and 74.96% for E. coli within 15 min. The limits of detection (LODs) of the platform were found to be 20 and 50 cells mL−1 for S. aureus and E. coli, respectively, and favorable linear relations were observed between the logarithm of bacterial concentration and the SERS intensity at the characteristic Raman peaks. The high specificity and potential clinical applications of the technique were also proven. Our method elucidated novel advances and potential applications in the field of multiple SERS detection of pathogens.

Graphical abstract: Sensitive and specific detection of clinical bacteria via vancomycin-modified Fe3O4@Au nanoparticles and aptamer-functionalized SERS tags

Supplementary files

Article information

Article type
Paper
Submitted
22 Feb 2018
Accepted
01 May 2018
First published
03 May 2018

J. Mater. Chem. B, 2018,6, 3751-3761

Sensitive and specific detection of clinical bacteria via vancomycin-modified Fe3O4@Au nanoparticles and aptamer-functionalized SERS tags

C. Zhang, C. Wang, R. Xiao, L. Tang, J. Huang, D. Wu, S. Liu, Y. Wang, D. Zhang, S. Wang and X. Chen, J. Mater. Chem. B, 2018, 6, 3751 DOI: 10.1039/C8TB00504D

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