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A simple design of fluorescent probe for indirect detection of β-lactamase based on AIE and ESIPT processes

Abstract

A novel fluorescent probe DNBS-CSA is developed for light-up detection of β-lactamase. The probe design is based on an indirect detection approach with three step reactions. β-Lactamase can react with the lactam of its substrate (cefazolin sodium) to produce a secondary amine, initiating a spontaneous elimination reaction and affording a thiol compound. The thiol could further react with the sulfonate group of DNBS-CSA, releasing the salicylaldehyde azine derivative (CSA) with both aggregation induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) characteristics. Previously reported β-lactamase probes require covalent linkage of the substrate β-lactam ring part to probe, which makes the probe synthesis difficult due to the complicated structure of β-lactam ring. On the contrary, modification of β-lactam ring is no longer necessary for DNBS-CSA according to our indirect detection approach. The linear range of fluorescent quantification for β-lactamase is 0-10 mU/mL in aqueous solution. Moreover, owing to the AIE property of CSA, detection of β-lactamase with DNBS-CSA on test papers was also achieved.

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Publication details

The article was received on 10 Feb 2018, accepted on 09 May 2018 and first published on 11 May 2018


Article type: Paper
DOI: 10.1039/C8TB00414E
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    A simple design of fluorescent probe for indirect detection of β-lactamase based on AIE and ESIPT processes

    L. Peng, L. Xiao, Y. Ding, Y. Xiang and A. Tong, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00414E

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