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RhBMP-2 and concomitant rapid material degradation synergistically promote bone repair and regeneration with collagen-hydroxyapatite nanocomposites

Abstract

The effective treatment of bone defects is still a great challenge in clinical practice. Synthetic bone-grafting substitutes of composition and structure analogue to bone as well as incorporated with growth factors are considered to be a promising solution. In this study, a collagen-hydroxyapatite (CHA) nanocomposite scaffold was developed by collagen self-assembly with simultaneous HA synthesis. The physicochemical properties such as morphology, inorganic phase, thermal decomposition, specific surface area and pore size distribution were characterized. The osteogenicity of CHA in the absence or presence of recombinant human bone morphogenetic protein-2 (rhBMP-2) was assessed both by cell culturing and animal implantation experiments. The gene expression results showed that the osteogenic differentiation capacity of rat bone mesenchymal stem cells (rBMSCs) have been enhanced both by CHA and rhBMP-2. The efficient bone regeneration of femoral defects in rabbits was achieved with CHA and CHA pre-absorbed rhBMP-2 (CHA/B), confirmed by micro-computed tomography measurement, histological observation and immunohistochemical analyses. The CHA nanocomposite was completely degraded within 8 weeks and replaced by new bone. It was found that rhBMP-2 not only accelerated and enhanced the bone formation, but also expedited the degradation of CHA. It is believed that the rhBMP-2 and concomitant rapid material degradation synergistically promote the bone repair and regeneration with CHA. The biodegradation behavior of CHA in the presence of rhBMP-2 can be further investigated to gain the in-depth understanding of the complex interplays among biomaterial, growth factor and their target cells. The relevant knowledge will facilitate seeking for the reasonable, safe and efficient methodology for introduction of growth factors to biomaterials so as to achieving the satisfied tissue regeneration.

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Publication details

The article was received on 09 Feb 2018, accepted on 04 Jun 2018 and first published on 05 Jun 2018


Article type: Paper
DOI: 10.1039/C8TB00405F
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    RhBMP-2 and concomitant rapid material degradation synergistically promote bone repair and regeneration with collagen-hydroxyapatite nanocomposites

    W. Su, X. Ma, Z. Sun, Z. Yi, X. Cui, G. Chen, X. Chen, B. Guo and X. Li, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00405F

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