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Reduction-responsive liposomal nanocarriar with self-reporting ability for efficient gene delivery

Abstract

In the past decades, although various reduction-responsive nanocarriers had been designed and explored for gene delivery, the reduction capability of the carriers were difficult to directly detect or monitor, especially in the intracellular condition. Taking advantage of the generated fluorescence signal in the reducing process of the naphthalimide-sulfonamide (NS) group, we developed a novel liposomal nanocarrier FNSL, which showed reduction-sensitive property and self-reporting character. As a new reduction-responsive site in gene delivery system, the NS group in FNSL is capable of responding to the glutathione (GSH) and simultaneously emitting green fluorescence at 500 nm both in extra- and intracellular circumstances. Hence, it will be very convenient to assess the reducibility of the carrier and monitor the stimuli-responsive gene release via the fluorescence signal. The FNSL have high affinity to DNA and can condense it into proper nano-size and zeta potential. By comparing with the non-reducible FNAL, FNSL showed enhanced gene release capability, higher transfection efficiency (TE) and lower cytotoxicity. Furthermore, the treatment of slight exogenous GSH largely improved the TE of FNSL in HepG2 cells, even higher than Lipofectamine 2000. These results demonstrate that FNSL possesses a great potential for efficient and low-toxic gene delivery and this work about bioreducible liposome with self-reporting ability would be a guide for further research about the development of biodegradable gene carriers.

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Publication details

The article was received on 03 Mar 2018, accepted on 06 Apr 2018 and first published on 07 Apr 2018


Article type: Paper
DOI: 10.1039/C8TB00392K
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Reduction-responsive liposomal nanocarriar with self-reporting ability for efficient gene delivery

    X. Yu, B. Wang, J. Zhang, Y. Liu, W. Zhang, Y. Xiao and R. Zhao, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00392K

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