A reduction-responsive liposomal nanocarrier with self-reporting ability for efficient gene delivery

Abstract

In the past few decades, although various reduction-responsive nanocarriers have been designed and explored for gene delivery, it is difficult to directly detect or monitor the reduction capability of these carriers, especially under intracellular conditions. Taking advantage of the generated fluorescence signal in the reduction process of the naphthalimide-sulfonamide (NS) group, we developed a novel liposomal nanocarrier, FNSL, which showed reduction-sensitive property and self-reporting character. As a new reduction-responsive site in a gene delivery system, the NS group in FNSL is capable of responding to glutathione (GSH) and simultaneously emitting green fluorescence at 500 nm in both extra- and intracellular circumstances. Hence, it will be very convenient to assess the reducibility of this carrier and monitor the stimuli-responsive gene release via fluorescence signal. FNSL has high affinity for DNA and can condense it into nanoparticles with a proper nano-size and zeta potential. Compared with the non-reducible FNAL, FNSL showed enhanced gene release capability, higher transfection efficiency (TE), and lower cytotoxicity. Furthermore, treatment of FNSL-mediated transfection with slightly exogenous GSH greatly improved the TE of FNSL in HepG2 cells, and its TE was even higher than that of Lipofectamine 2000. These results demonstrate that FNSL possesses great potential for efficient and low-toxicity gene delivery, and this study on a bioreducible liposome with self-reporting ability would be a guide for further research on the development of biodegradable gene carriers.