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In-situ polymerization of poly(γ-benzyl-L-glutamate) on mesoporous hydroxyapatite with high graft amounts for direct fabrication of biodegradable cell microcarriers and its osteogenesis induction

Abstract

Large-scale cell culture for cell expansion in tissue engineering is currently a major focus of research. One method to achieve better cell amplification is to utilize microcarriers. In this study, Different amounts of poly(γ-benzyl-L-glutamate) (PBLG) (from 11 wt% to 50 wt%) were grafted on the mesoporous hydroxyapatite (MHA) by the in-situ ring opening polymerization of γ-benzyl-L-glutamate N-carboxyanhydride (BLG-NCA) and the biodegradable and biocompatible PBLG-g-MHA microcarriers were directly fabricate using the oil-in-water (O/W) solvent-evaporation technique for bone tissue engineering. The amount of grafted PBLG could be controlled by adjusting the feed ratio of MHA and BLG-NCA. The relationships between sphere morphology and graft amount or solution concentration were explored. Furthermore, cytological assays were performed to evaluate the biological properties of PBLG-g-MHA microcarriers. For a solution concentration of 3% (w/V) and PBLG graft amounts of 33 wt% and 50 wt%, the microspheres could be harvested with optimal spherical shapes. In vitro cell culture revealed that the PBLG-g-MHA microspheres had favorable properties for cell proliferation and significantly enhanced the osteogenic differentiation of MC3T3-E1 cells and bone matrix formation.

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Publication details

The article was received on 25 Jan 2018, accepted on 10 Apr 2018 and first published on 12 Apr 2018


Article type: Paper
DOI: 10.1039/C8TB00232K
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    In-situ polymerization of poly(γ-benzyl-L-glutamate) on mesoporous hydroxyapatite with high graft amounts for direct fabrication of biodegradable cell microcarriers and its osteogenesis induction

    L. Li, X. Shi, Z. Wang, Y. Wang, Z. Jiao and P. Zhang, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00232K

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