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Issue 12, 2018
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pH-Independent two-photon fluorescent lysotrackers for real-time monitoring autophagy

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Abstract

Two carbazole-based two-photon fluorescent lysotrackers with different electron-donating groups (a methoxyl group for Lyso-MCO and a dimethylamino group for Lyso-NCO, respectively) have been developed from simple starting materials via an only 2-step procedure. Both of them exhibit pH-independent and specific lysosome location with a rapid staining rate, high photostability and deep issue penetration along with large two-photon absorption action cross-sections. By virtue of the better two-photon absorption properties of Lyso-NCO, it was chosen to visually monitor lysosomal tracking and autophagy. Compared with the approach of GFP-LC3 for autophagy detection, lysotracker Lyso-NCO achieved efficient and real-time visualization of the membrane fusion period in the autophagy process through detecting the level of the co-localization coefficients between Lyso-NCO and Mitotracker Red FM (MTR) in live cells.

Graphical abstract: pH-Independent two-photon fluorescent lysotrackers for real-time monitoring autophagy

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Publication details

The article was received on 25 Jan 2018, accepted on 14 Feb 2018 and first published on 14 Feb 2018


Article type: Paper
DOI: 10.1039/C8TB00229K
Citation: J. Mater. Chem. B, 2018,6, 1764-1770
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    pH-Independent two-photon fluorescent lysotrackers for real-time monitoring autophagy

    W. Wang, P. Ning, Q. Wang, W. Zhang, J. Jiang, Y. Feng and X. Meng, J. Mater. Chem. B, 2018, 6, 1764
    DOI: 10.1039/C8TB00229K

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