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Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P22: covalent loading and cleavable release

Abstract

Peptides have great potentials in the treatment of various diseases because of high specificity and safety. However, their application has been limited by the lack of appropriate delivery techniques. In this study we have demonstrated the intracellular delivery of peptides using the viral nanoparticles (VNPs) of bacteriophage P22 through covalent loading of two different peptides with synergistic cytotoxic effects and controllable release triggered by Cathepsin B, a highly over-expressed protease in many tumors. Fluorescence imaging of the intracellular localization of P22 VNPs and the peptides evidenced that the delivery system could be transported as designed. CCK8 assay and flow cytometry analysis revealed the cytotoxic effects of the peptides on the MD-MBA-231 tumor cells. These results have confirmed the feasibility of genetically fusing cargo peptides to the capsid protein of P22 and cleavable release thereafter. This study may be instructive for the design of drug delivery systems and multifunctional theranostic devices based on P22 VNPs.

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Publication details

The article was received on 21 Jan 2018, accepted on 10 May 2018 and first published on 10 May 2018


Article type: Paper
DOI: 10.1039/C8TB00186C
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P22: covalent loading and cleavable release

    J. Wang, T. Fang, M. Li, W. Zhang, Z. Zhang, X. Zhang and F. Li, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00186C

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