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Glass transition in temperature-responsive poly(butyl methacrylate) grafted polymer brushes. Impact of thickness and temperature on wetting and morphology, cell growth

Abstract

Poly(n-butyl methacrylate) – (PBMA) grafted polymer brushes attached to glass were fabricated in a three-step process involving surface initiated atom transfer radical polymerization. The surface properties of the coatings after subsequent fabrication steps were confirmed using ToF-SIMS and ellipsometry. Measurements of water contact angle and AFM revealed temperature-induced changes in hydrophobicity and morphology of the coating. The glass transition temperatures (Tg) of the PBMA coatings with different thickness were determined from AFM measurements. For PBMA grafted brush coatings with thicknesses less than 62 nm Tg increases sharply with increasing thickness. PBMA grafted coatings of thickness equal to 86 nm and 43 nm as well as control glass substrates were used as substrates for culturing an urinary bladder cancer HTB-5 cell line. After 144h of culturing, well-developed monocellular layer may be observed on the PBMA-coating of thickness equal to 86 nm. In turn, cells incubated on thinner (43 nm) PBMA-coating as well as on control glass sample only start to form confluent layer.

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Publication details

The article was received on 10 Jan 2018, accepted on 09 Feb 2018 and first published on 09 Feb 2018


Article type: Paper
DOI: 10.1039/C8TB00088C
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Glass transition in temperature-responsive poly(butyl methacrylate) grafted polymer brushes. Impact of thickness and temperature on wetting and morphology, cell growth

    Y. Stetsyshyn, J. Raczkowska, O. Lishchynskyi, K. Awsiuk, J. Zemla, P. Dąbczyński, A. Kostruba, K. Harhay, H. Ohar, B. Orzechowska, Y. Panchenko, P. Vankevych and A. Budkowski, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB00088C

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