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Quantitatively monitoring oxygen variation in Endoplasmic Reticulum with a fluorophore-phosphor energy transfer cassette

Abstract

An efficient excitation energy transfer (EET) cassette, i.e. Ir-Np-OH, has been constructed by connecting an Ir(III) complex phosphor as acceptor to a naphthalimide fluorophore as donor. Ir-Np-OH emits dual emissions, with one highly dependent on oxygen concentration and the other completely independent. Thus, Ir-Np-OH exhibits a sensitive and quantitative response to oxygen level in a ratiometric way. The linear response of Ir-Np-OH ranges from 0% (0 mmHg) to 10% (76 mmHg) oxygen tension, which matches very well with intracellular oxygen level. And the ratiometric oxygen sensing of Ir-Np-OH is completely reversible. Also importantly, Ir-Np-OH possesses a subcellular targetability specifically toward endoplasmic reticulum (ER), as proved by the co-localization imaging experiment. This feature of Ir-Np-OH should be noted, because, generally speaking, it is much more challenging for small-molecule probes to target ER than other subcellular organelles, e.g. mitochondria. By using Ir-Np-OH, an apparent increase in oxygen level from 5.17 % (39.29 mmHg) to 7.29 % (55.40 mmHg) is monitored in ER of live MCF-7 cells, during the 30-minute stimulation with Rotenone, a typical inhibitor of mitochondrial respiratory chain. In addition, upon ER stress directly induced by TG (Thapsigargin), the gradual increase in oxygen concentration is also clearly detected. These results confirm that Ir-Np-OH is a promising tool for oxygen-related cellular and chemical biology.

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Publication details

The article was received on 20 Dec 2017, accepted on 13 Feb 2018 and first published on 15 Feb 2018


Article type: Paper
DOI: 10.1039/C7TB03279J
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Quantitatively monitoring oxygen variation in Endoplasmic Reticulum with a fluorophore-phosphor energy transfer cassette

    H. Bian, X. Song, N. Li, H. Man and Y. Xiao, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C7TB03279J

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