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Issue 15, 2018
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The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

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Abstract

Nanoparticles offer a promising avenue for the targeted delivery of therapies. To slow clearance, nanoparticles are frequently stealth-coated to prevent opsonization and immune recognition. Serum albumin (SA) has been used as a bio-inspired stealth coating. To develop this shielding strategy for clinical applications, it is critical to understand the interactions between the immune system and SA-camouflaged nanoparticles. This work investigates the in vivo processing of SA-coated nanoparticles using tobacco mosaic virus (TMV) as a model system. In comparing four different SA-formulations, the particles with high SA coverage conjugated to TMV via a short linker performed the best at preventing antibody recognition. All formulations led to similar levels of TMV-specific antibodies after repeat administration in mice; importantly though, SA-specific antibodies were not detected and the TMV-specific antibodies were unable to recognize shielded SA-coated TMV. Upon uptake in macrophages, the shielding agent and nanoparticle separate, where TMV trafficked to the lysosome and SA appears to recycle. The distinct intracellular fates of the TMV carrier and SA shielding agent explain why anti-TMV but not SA-specific antibodies are generated. This work characterizes the outcomes of SA-camouflaged TMV after immune recognition, and highlights the effectiveness of SA as a nanoparticle shielding agent.

Graphical abstract: The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

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Publication details

The article was received on 30 Nov 2017, accepted on 21 Feb 2018 and first published on 27 Feb 2018


Article type: Paper
DOI: 10.1039/C7TB03106H
Citation: J. Mater. Chem. B, 2018,6, 2204-2216
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    The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition

    N. M. Gulati, A. S. Pitek, A. E. Czapar, P. L. Stewart and N. F. Steinmetz, J. Mater. Chem. B, 2018, 6, 2204
    DOI: 10.1039/C7TB03106H

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