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Inflammatory activation of human serum albumin- or ovalbumin-modified chitosan nanoparticles to macrophages and their immune response in human whole blood

Abstract

The nanomaterials have been extensively applied in biomedical field. These nanoscale objects may either promote or restrain immune responses depending on their surface characteristics and compositions. In this study, chitosan (CS) particles prepared by an emulsion-crosslinking method were modified with different amounts of human serum albumin (HSA) and ovalbumin (OVA), resulting in four types of modified CS particles, i.e. CS@HSA-10, CS@HSA-57, CS@OVA-13 and CS@OVA-65, respectively. They had a similar size of about 150 nm in a dry state, and were swollen to 2-3 folds in PBS. No significant cytotoxicity was determined to the in vitro cultured RAW264.7 and THP-1 cells. However, all the modified CS particles, in particular the OVA-modified ones (CS@OVA-13 and CS@OVA-65), induced significantly higher secretion of tumor necrosis factor-α (TNF-) and interleukin-6 (IL-6) compared with the negative control. In human whole blood, the CS@OVA-13 and CS@OVA-65 were phagocytosed with a significantly higher ratio by the granulocytes and monocytes, leading to the larger secretion of TNF-, IL-1 and IL-8, and a larger extent of platelet activation than the CS@HSA-10 and CS@HSA-57, respectively.

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Publication details

The article was received on 29 Nov 2017, accepted on 05 Apr 2018 and first published on 10 Apr 2018


Article type: Paper
DOI: 10.1039/C7TB03096G
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Inflammatory activation of human serum albumin- or ovalbumin-modified chitosan nanoparticles to macrophages and their immune response in human whole blood

    C. Gao, Y. Zhang, D. Wang, K. Smuda, R. Georgieva and H. Bäumler, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C7TB03096G

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