Jump to main content
Jump to site search

Issue 9, 2018
Previous Article Next Article

A reversible frequency upconversion probe for real-time intracellular lysosome-pH detection and subcellular imaging

Author affiliations

Abstract

Lysosomal pH is known to be acidic, which plays a vital role in various processes in living organisms. Frequency upconversion luminescence exhibits the unique ability to convert low energy photons into high energy photons, which can be used in live samples due to its deep penetration, low tissue damage, etc. The development of sensitive frequency upconversion probes with a low pKa, high sensitivity, and specific selectivity for lysosomal pH detection is of great importance. Herein, we report a novel frequency upconversion luminescence probe, NRH-Lyso, by conjugating the rhodamine derivative NRH and the lysosome targeting functional group 4-(2-aminoethyl)morpholine. NRH-Lyso shows a sensitive response to acidic pH and excellent selectivity in the presence of metal ions, anions, and small molecules. Due to the structural transformation of lactam ring opening and closing of NRH-Lyso, the probe shows an almost 80-fold enhancement in emission intensity when the pH changes from 7.0 to 4.0 under 808 nm laser excitation. The living cell imaging data reveals that NRH-Lyso can selectively detect lysosomal pH changes with excellent photostability and low cytotoxicity. All these features make NRH-Lyso a good candidate to investigate the lysosomal pH-associated physiological and pathological processes.

Graphical abstract: A reversible frequency upconversion probe for real-time intracellular lysosome-pH detection and subcellular imaging

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 Nov 2017, accepted on 26 Jan 2018 and first published on 09 Feb 2018


Article type: Paper
DOI: 10.1039/C7TB03089D
Citation: J. Mater. Chem. B, 2018,6, 1322-1327
  •   Request permissions

    A reversible frequency upconversion probe for real-time intracellular lysosome-pH detection and subcellular imaging

    Z. Dong, Q. Han, Z. Mou, G. Li and W. Liu, J. Mater. Chem. B, 2018, 6, 1322
    DOI: 10.1039/C7TB03089D

Search articles by author

Spotlight

Advertisements