Jump to main content
Jump to site search

Issue 18, 2018
Previous Article Next Article

Covalent functionalization of graphene oxide with D-mannose: evaluating the hemolytic effect and protein corona formation

Author affiliations

Abstract

In this work, graphene oxide (GO) was covalently functionalized with D-mannose (man-GO) using mannosylated ethylenediamine. XPS (C1s and N1s) confirmed the functionalization of GO through the binding energies at 288.2 eV and 399.8 eV, respectively, which are attributed to the amide bond. ATR-FTIR spectroscopy showed an increase in the amine bond intensity, at 1625 cm−1 (stretching C[double bond, length as m-dash]O), after the functionalization step. Furthermore, the man-GO toxicity to human red blood cells (hemolysis) and its nanobiointeractions with human plasma proteins (hard corona formation) were evaluated. The mannosylation of GO drastically reduced its toxicity to red blood cells. SDS-PAGE analysis showed that the mannosylation process of GO also drastically reduced the amount of the proteins in the hard corona. Additionally, proteomics analysis by LC–MS/MS revealed 109 proteins in the composition of the man-GO hard corona. Finally, this work contributes to future biomedical applications of graphene-based materials functionalized with active biomolecules.

Graphical abstract: Covalent functionalization of graphene oxide with d-mannose: evaluating the hemolytic effect and protein corona formation

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Nov 2017, accepted on 08 Apr 2018 and first published on 10 Apr 2018


Article type: Paper
DOI: 10.1039/C7TB02997G
Citation: J. Mater. Chem. B, 2018,6, 2803-2812
  •   Request permissions

    Covalent functionalization of graphene oxide with D-mannose: evaluating the hemolytic effect and protein corona formation

    M. de Sousa, C. H. Z. Martins, L. S. Franqui, L. C. Fonseca, F. S. Delite, E. M. Lanzoni, D. S. T. Martinez and O. L. Alves, J. Mater. Chem. B, 2018, 6, 2803
    DOI: 10.1039/C7TB02997G

Search articles by author

Spotlight

Advertisements