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Alginate dialdehyde meets nylon membrane: A versatile platform for facile and green fabrication of membrane adsorbers

Abstract

Alginate dialdehyde (ADA), a biocompatible polymer, was used as an intermediate layer on nylon membrane to readily fabricate cation exchange (CEX), metal-affinity (Me-affinity), histidine-affinity (His-Affinity) and peptide-affinity (Pep-affinity) membrane adsorbers without any organic solvent usage. All the membrane adsorbers exhibited a high selectivity in fractionation of IgG (immunoglobulin)/HSA (human serum albumin) mixture. Along with a high purity of 100%, a high IgG binding capacity of 30, 24, 21 and 28 mg/mL (membrane volume) was achieved by CEX, Me-affinity, His-affinity and Pep-affinity membrane adsorbers in flow-through mode respectively, which is superior to the reported membrane adsorbers. Furthermore, the CEX and Pep-affinity adsorbers were tested for the separation of IgG from human plasma solution. The carboxylic groups along with the peptides on Pep-affinity adsorber captured IgG synergistically with higher recovery and purity (99 % and 98.6 %). This synergetic adsorber showed a qmax and dynamic binding capacity (DBC) of 138.4 and 38.7 mg/mL respectively for IgG binding which was quite higher than the reported Protein A agarose (having qmax and DBC of 84 and 24.4 mg/g respectively). The present work indicated that the ADA layer not only activated membrane surface to attach various adsorptive ligands under mild conditions, but also reduced non-specific adsorption. Due to the versatile linking function and green reaction conditions, the ADA coating on nylon membrane is promising for the preparation of diverse membrane adsorbers.

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Publication details

The article was received on 14 Nov 2017, accepted on 06 Feb 2018 and first published on 08 Feb 2018


Article type: Paper
DOI: 10.1039/C7TB02966G
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Alginate dialdehyde meets nylon membrane: A versatile platform for facile and green fabrication of membrane adsorbers

    M. K. Khan, J. Luo, Z. Wang, R. Khan, X. Chen and Y. Wan, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C7TB02966G

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