Jump to main content
Jump to site search


A chemically induced proximity system engineered from the plant auxin signaling pathway

Author affiliations

Abstract

Methods based on chemically induced proximity (CIP) serve as powerful tools to control cellular processes in a temporally specific manner. To expand the repertoire of CIP systems available for studies of cellular processes, we engineered the plant auxin signaling pathway to create a new indole-3-acetic acid (IAA) based CIP method. Auxin-induced protein degradation that occurs in the natural pathway was eliminated in the system. The new IAA based method is both readily inducible and reversible, and used to control the production of therapeutic proteins that induced the apoptosis of cancer cells. The approach is also orthogonal to existing CIP systems and used to construct a biological Boolean logic gate controlling gene expression system. We believe that the new CIP method will be applicable to the artificial control and dissection of complex cellular functions.

Graphical abstract: A chemically induced proximity system engineered from the plant auxin signaling pathway

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 May 2018, accepted on 09 Jun 2018 and first published on 12 Jun 2018


Article type: Edge Article
DOI: 10.1039/C8SC02353K
Citation: Chem. Sci., 2018, Advance Article
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    A chemically induced proximity system engineered from the plant auxin signaling pathway

    W. Zhao, H. Nguyen, G. Zeng, D. Gao, H. Yan and F. Liang, Chem. Sci., 2018, Advance Article , DOI: 10.1039/C8SC02353K

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements