Jump to main content
Jump to site search


A multifunctional SERS sticky note for real-time quorum sensing tracing and inactivation of bacterial biofilm

Abstract

Quorum sensing (QS) is crucial for bacterial survival and activity. Although detecting related signaling metabolites can reveal the QS, a versatile platform for convenient real-time imaging of their secretion in the context of bacterial biofilms along with the inhibition function to the growth of biofilms is still highly desired. Here we develop a flexible sticky note with sandwich structure by encapsulating gold nanostars between two pieces of hexagonal boron nitride layers, which can be easily pasted on natural biofilm to real-time monitor the secreted signaling molecule by SERS imaging with high sensitivity and spatiotemporal resolution. Using Pseudomonas aeruginosa and its pyocyanin secretion as model and an internal standard for self-calibration of SERS signal, the sticky note achieves reliable quantification and rapid response to the secretion as early as 1-h biofilm growth. With antibiotic loading, the multifunctional SERS sticky note also demonstrated effective inactivation of bacterial biofilm with simultaneous evaluation of the inactivation effect. This multifunctional SERS sticky note provides a versatile platform in investigating bacterial behaviors and developing antibacterial therapeutics.

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 May 2018, accepted on 30 May 2018 and first published on 31 May 2018


Article type: Edge Article
DOI: 10.1039/C8SC02078G
Citation: Chem. Sci., 2018, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    A multifunctional SERS sticky note for real-time quorum sensing tracing and inactivation of bacterial biofilm

    H. Ju, J. Guo, Y. Liu, Y. Chen and J. Li, Chem. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8SC02078G

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements