Jump to main content
Jump to site search

Issue 21, 2018
Previous Article Next Article

A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli

Author affiliations

Abstract

Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and L-cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.

Graphical abstract: A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Mar 2018, accepted on 02 May 2018 and first published on 03 May 2018


Article type: Edge Article
DOI: 10.1039/C8SC01130C
Citation: Chem. Sci., 2018,9, 4730-4735
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli

    N. Jiang, G. Ying, A. K. Yetisen, Y. Montelongo, L. Shen, Y. Xiao, H. J. Busscher, X. Yang and B. Su, Chem. Sci., 2018, 9, 4730
    DOI: 10.1039/C8SC01130C

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements