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Issue 25, 2018
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Genetically encoded tags for real time dissection of protein assembly in living cells

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Abstract

Simple methods with straightforward readouts that enable real-time interrogation of protein quaternary structure are much needed to facilitate the physicochemical characterization of proteins at the single-cell level. After screening over a series of microtubule (MT) binders, we report herein the development of two genetically encoded tags (designated as “MoTags” for the monomer/oligomer detection tag) that can be conveniently fused to a given protein to probe its oligomeric state in cellulo when combined with routine fluorescence microscopy. In their monomeric form, MoTags are evenly distributed in the cytosol; whereas oligomerization enables MoTags to label MT or track MT tips in an oligomeric state-dependent manner. We demonstrate here the broad utility of engineered MoTags to aid the determination of protein oligomeric states, dissection of protein structure and function, and monitoring of protein–target interactions under physiological conditions in living cells.

Graphical abstract: Genetically encoded tags for real time dissection of protein assembly in living cells

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Publication details

The article was received on 21 Feb 2018, accepted on 22 May 2018 and first published on 23 May 2018


Article type: Edge Article
DOI: 10.1039/C8SC00839F
Citation: Chem. Sci., 2018,9, 5551-5555
  • Open access: Creative Commons BY-NC license
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    Genetically encoded tags for real time dissection of protein assembly in living cells

    G. Ma, Q. Zhang, L. He, N. T. Nguyen, S. Liu, Z. Gong, Y. Huang and Y. Zhou, Chem. Sci., 2018, 9, 5551
    DOI: 10.1039/C8SC00839F

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