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Issue 14, 2018
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Development of microfluidic platforms for the synthesis of metal complexes and evaluation of their DNA affinity using online FRET melting assays

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Abstract

Guanine-rich DNA sequences can fold into quadruple-stranded structures known as G-quadruplexes. These structures have been proposed to play important biological roles and have been identified as potential drug targets. As a result, there is increasing interest in developing small molecules that can bind to G-quadruplexes. So far, these efforts have been mostly limited to conventional batch synthesis. Furthermore, no quick on-line method to assess new G-quadruplex binders has been developed. Herein, we report on two new microfluidic platforms to: (a) readily prepare G-quadruplex binders (based on metal complexes) in flow, quantitatively and without the need for purification before testing; (b) a microfluidic platform (based on FRET melting assays of DNA) that enables the real-time and on-line assessment of G-quadruplex binders in continuous flow.

Graphical abstract: Development of microfluidic platforms for the synthesis of metal complexes and evaluation of their DNA affinity using online FRET melting assays

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Publication details

The article was received on 01 Feb 2018, accepted on 28 Feb 2018 and first published on 01 Mar 2018


Article type: Edge Article
DOI: 10.1039/C8SC00528A
Citation: Chem. Sci., 2018,9, 3459-3469
  • Open access: Creative Commons BY-NC license
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    Development of microfluidic platforms for the synthesis of metal complexes and evaluation of their DNA affinity using online FRET melting assays

    V. Rakers, P. Cadinu, J. B. Edel and R. Vilar, Chem. Sci., 2018, 9, 3459
    DOI: 10.1039/C8SC00528A

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