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Multiple advanced logic gates made of DNA-Ag nanocluster and the application for intelligent detection of pathogenic bacterial genes

Abstract

Integration of multiple DNA logic gates on a universal platform to implement advance logic functions is a critical challenge for DNA computing. In the present investigation, a straightforward and powerful strategy of guanine-rich DNA sequence lighting up silver nanocluster and fluorophore was developed to construct a library of logic gates on a simple DNA-templated silver nanoclusters (DNA-AgNCs) platform. This library included basic logic gates, YES, AND, OR, INHIBIT, XOR, which were further integrated into complex logic circuits to implement diverse advanced arithmetic/non-arithmetic functions including half-adder, half-subtractor, multiplexer, and demultiplexer. Under UV irradiation, all logic functions could be instantly visualized, confirming excellent repeatability. The logic operations were entirely based on DNA hybridization in an enzyme-free and label-free condition, avoiding waste accumulation and reducing cost consumption. Interestingly, DNA-AgNCs based multiplexer was for the first time used as intelligent biosensor to identify pathogenic genes, E. coli and S. aureus genes, with high sensitivity. The investigation provides a prototype for wireless integration of multiple devices on even the simplest single-strand DNA platform to perform diverse complex functions in a straightforward and cost-effective way.

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Publication details

The article was received on 11 Dec 2017, accepted on 05 Jan 2018 and first published on 08 Jan 2018


Article type: Edge Article
DOI: 10.1039/C7SC05246D
Citation: Chem. Sci., 2018, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
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    Multiple advanced logic gates made of DNA-Ag nanocluster and the application for intelligent detection of pathogenic bacterial genes

    Y. Liu, X. Lin, J. Deng, Y. Lyu, P. Qian, Y. Li and S. Wang, Chem. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C7SC05246D

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