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Issue 8, 2018
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Ultrasensitive and specific fluorescence detection of a cancer biomarker via nanomolar binding to a guanidinium-modified calixarene

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Abstract

We designed a water-soluble guanidinium-modified calix[5]arene to target lysophosphatidic acid (LPA), an ideal biomarker for early diagnosis of ovarian and other gynecologic cancers, achieving binding on the nanomolar level. An indicator displacement assay, coupled with differential sensing, enabled ultrasensitive and specific detection of LPA. Moreover, we show that using a calibration line, the LPA concentration in untreated serum can be quantified in the biologically relevant low μM range with a detection limit of 1.7 μM. The reported approach is feasible for diagnosing ovarian and other gynecologic cancers, particularly at their early stages.

Graphical abstract: Ultrasensitive and specific fluorescence detection of a cancer biomarker via nanomolar binding to a guanidinium-modified calixarene

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Publication details

The article was received on 21 Nov 2017, accepted on 08 Jan 2018 and first published on 09 Jan 2018


Article type: Edge Article
DOI: 10.1039/C7SC04989G
Citation: Chem. Sci., 2018,9, 2087-2091
  • Open access: Creative Commons BY license
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    Ultrasensitive and specific fluorescence detection of a cancer biomarker via nanomolar binding to a guanidinium-modified calixarene

    Z. Zheng, W. Geng, J. Gao, Y. Wang, H. Sun and D. Guo, Chem. Sci., 2018, 9, 2087
    DOI: 10.1039/C7SC04989G

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