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Issue 10, 2018
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Label-free and live cell imaging by interferometric scattering microscopy

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Abstract

Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label. Here we report label-free and live-cell imaging of mammalian cell, Escherischia coli, and yeast, using interferometric scattering microscopy, which reveals the underlying structures of a variety of cytoplasmic organelles as well as the underside structure of the cells. The contact areas of the cells attached onto a glass substrate, e.g., focal adhesions and filopodia, are clearly discernible. We also found a variety of fringe-like features in the cytoplasmic area, which may reflect the folded structures of cytoplasmic organelles. We thus anticipate that the label-free interferometric scattering microscopy can be used as a powerful tool to shed interferometric light on in vivo structures and dynamics of various intracellular phenomena.

Graphical abstract: Label-free and live cell imaging by interferometric scattering microscopy

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Publication details

The article was received on 02 Nov 2017, accepted on 08 Feb 2018 and first published on 09 Feb 2018


Article type: Edge Article
DOI: 10.1039/C7SC04733A
Citation: Chem. Sci., 2018,9, 2690-2697
  • Open access: Creative Commons BY-NC license
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    Label-free and live cell imaging by interferometric scattering microscopy

    J. Park, I. Lee, H. Moon, J. Joo, K. Kim, S. Hong and M. Cho, Chem. Sci., 2018, 9, 2690
    DOI: 10.1039/C7SC04733A

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