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Issue 14, 2018
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On-chip measurements of protein unfolding from direct observations of micron-scale diffusion

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Abstract

Investigations of protein folding, unfolding and stability are critical for the understanding of the molecular basis of biological structure and function. We describe here a microfluidic approach to probe the unfolding of unlabelled protein molecules in microliter volumes. We achieve this objective through the use of a microfluidic platform, which allows the changes in molecular diffusivity upon folding and unfolding to be detected directly. We illustrate this approach by monitoring the unfolding of bovine serum albumin in solution as a function of pH. These results show the viability of probing protein stability on chip in small volumes.

Graphical abstract: On-chip measurements of protein unfolding from direct observations of micron-scale diffusion

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Publication details

The article was received on 05 Oct 2017, accepted on 09 Feb 2018 and first published on 09 Feb 2018


Article type: Edge Article
DOI: 10.1039/C7SC04331G
Citation: Chem. Sci., 2018,9, 3503-3507
  • Open access: Creative Commons BY license
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    On-chip measurements of protein unfolding from direct observations of micron-scale diffusion

    Y. Zhang, Emma V. Yates, L. Hong, K. L. Saar, G. Meisl, C. M. Dobson and T. P. J. Knowles, Chem. Sci., 2018, 9, 3503
    DOI: 10.1039/C7SC04331G

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