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Issue 8, 2018
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Detection of antimicrobial resistance-associated proteins by titanium dioxide-facilitated intact bacteria mass spectrometry

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Abstract

Titanium dioxide-modified target plates were developed to enhance intact bacteria analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The plates were designed to photocatalytically destroy the bacterial envelope structure and improve the ionization efficiency of intracellular components, thereby promoting the measurable mass range and the achievable detection sensitivity. Accordingly, a method for rapid detection of antimicrobial resistance-associated proteins, conferring bacterial resistance against antimicrobial drugs, was established by mass spectrometric fingerprinting of intact bacteria without the need for any sample pre-treatment. With this method, the variations in resistance proteins’ expression levels within bacteria were quickly measured from the relative peak intensities. This approach of resistance protein detection directly from intact bacteria by mass spectrometry is useful for fast discrimination of antimicrobial-resistant bacteria from their non-resistant counterparts whilst performing species identification. Also, it could be used as a rapid and convenient way for initial determination of the underlying resistance mechanisms.

Graphical abstract: Detection of antimicrobial resistance-associated proteins by titanium dioxide-facilitated intact bacteria mass spectrometry

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Publication details

The article was received on 18 Sep 2017, accepted on 18 Jan 2018 and first published on 18 Jan 2018


Article type: Edge Article
DOI: 10.1039/C7SC04089J
Citation: Chem. Sci., 2018,9, 2212-2221
  • Open access: Creative Commons BY license
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    Detection of antimicrobial resistance-associated proteins by titanium dioxide-facilitated intact bacteria mass spectrometry

    Y. Zhu, N. Gasilova, M. Jović, L. Qiao, B. Liu, L. T. Lovey, H. Pick and H. H. Girault, Chem. Sci., 2018, 9, 2212
    DOI: 10.1039/C7SC04089J

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