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Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification

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Abstract

Lipase immobilised on silica monoliths has been prepared and applied as biocatalytic continuous-flow microreactors for the transesterification of tributyrin as a model bio-oil component. Candida antarctica lipase was trapped within the pores of silica monoliths, and its successful immobilisation was demonstrated by the hydrolysis of 4-nitrophenyl butyrate to 4-nitrophenol. Lipase immobilised on silica monoliths was active for the transesterification of tributyrin at ambient temperature, with reactivity as a function of the methanol : tributyrin ratio, flow rate, temperature, and textural properties. Monoliths with a high surface area and large meso- and macropore channels enhanced the transesterification activity through improved molecule diffusion. The optimum immobilised lipase microreactor exhibited almost quantitative ester production for >100 h at 30 °C without deactivation.

Graphical abstract: Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification

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Publication details

The article was received on 02 Oct 2017, accepted on 18 Dec 2017 and first published on 18 Dec 2017


Article type: Paper
DOI: 10.1039/C7RE00162B
Citation: React. Chem. Eng., 2018, Advance Article
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    Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification

    M. Alotaibi, J. C. Manayil, G. M. Greenway, S. J. Haswell, S. M. Kelly, A. F. Lee, K. Wilson and G. Kyriakou, React. Chem. Eng., 2018, Advance Article , DOI: 10.1039/C7RE00162B

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