Eugenol triggers CD11b+Gr1+ myeloid-derived suppressor cell apoptosis via endogenous apoptosis pathway

Ying Ding; Zecheng Yang; Wensheng Zhang; Yuwei Xu; Yuanyuan Wang; Minghua Hu; Fangli Ma; Hanan Long; Ning Tao; Zhihai Qin

doi:10.1039/C7RA13499A

Eugenol triggers CD11b⁺Gr1⁺ myeloid-derived suppressor cell apoptosis via endogenous apoptosis pathway

Ying Ding,

†^a Zecheng Yang,†^b Wensheng Zhang,

^c Yuwei Xu,

^b Yuanyuan Wang,

^d Minghua Hu,

^d Fangli Ma,

^d Hanan Long,*^fg Ning Tao

*^e and Zhihai Qin

*^ae

Author affiliations

* Corresponding authors

^a School of Basic Medical Sciences of Southwest Medical University, Luzhou, China

^b College of Life Science, University of the Chinese Academy of Sciences, Beijing, China

^c Department of Microbiology and Immunology, Shanxi Medical University, Taiyuan, China

^d Infinitus Chinese Herbal Immunity Research Centre, Infinitus China Company Ltd, Guangzhou, China

^e Key Laboratory of Protein and Peptide Pharmaceuticals, Institute of Biophysics, Chinese Academy of Sciences, Datun Road No. 15, Chaoyang District, Beijing, China
E-mail: tao@ibp.ac.cn, zhihai@ibp.ac.cn

^f Department of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China
E-mail: longhanan@swmu.edu.cn

^g Department of Science and Technology, Southwest Medical University, Luzhou, Sichuan, China

Abstract

To study the effect and underlying molecular mechanism of eugenol on CD11b⁺Gr1⁺ myeloid-derived suppressor cells (MDSCs). The effect of eugenol on the inhibition of immortalized MDSC cell line MSC-2 and murine peritoneal macrophages was detected by MTT. Flow cytometry was used to detect the pro-apoptosis effect of eugenol on MDSCs. The expression levels of apoptosis-related proteins were detected by western blot. Eugenol has a selective inhibitory effect on MDSCs in a dose-dependent manner, which activates an endogenous apoptosis pathway, leading to apoptosis. Eugenol promotes the apoptosis of MDSCs via the intrinsic pathway.

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Eugenol triggers CD11b⁺Gr1⁺ myeloid-derived suppressor cell apoptosis via endogenous apoptosis pathway

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Eugenol triggers CD11b⁺Gr1⁺ myeloid-derived suppressor cell apoptosis via endogenous apoptosis pathway

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